AAT Bioquest公司熒光探針Fluo-8®使用說明

 蘇州螞蟻淘生物科技有限公司是AATBIO的中國大陸區(qū)授權代理商

產(chǎn)品介紹：       

         染料DiI, DiO, DiD 和 DiR是一類親脂性熒光染料家族，用于標記細胞膜和疏水性組織。這是一類環(huán)境敏感型熒光染料，當它們與膜結合或者與親脂性生物分子(例如蛋白質，雖然在水中其熒光強度很弱)結合時，其熒光強度顯著增強。它們具有很高的淬滅系數(shù)，偏光依賴性和很短的激發(fā)壽命。一旦應用于細胞中，這種染料會在細胞內質膜中逐步擴散，導致在其*濃度條件下，將整個細胞染色。

        它們不同的熒光顏色：DiI（橙色熒光）、DiO（綠色熒光）、DiD（紅色熒光）、DiR（深紅色熒光），為活細胞多色彩熒光成像分析和流式細胞術提供了一種便捷的工具。DiO和DiI可以分別與標準的FITC和TRITC濾光器一同使用，其中，DiO可以被633 nm He-Ne激光激發(fā)，并且具有比DiI更長的激發(fā)和發(fā)射光波長，為標記細胞和組織的那些本身就具有本底熒光的染料提供了非常的替代品。

        DiR在活體成像或者示蹤中非常有用，因為它們所發(fā)射的紅外光可以高效地穿過細胞和組織，并且在紅外光范圍內，其本底熒光水平很低。

技術資料：

      1.Ex(nm)：748

      2.Em(nm)：780

      3.分子量：

      4.溶劑：DMSO

AAT Bioquest公司熒光探針Fluo-8®使用說明

 Fluo-8® AM is a novel green fluorescent indicator that utilizes a fluorescein core to monitor Ca2+ concentration and flux in cells. It has several key advantages over earlier generations of indicators like Fluo-3 AM and Fluo-4 AM:

• Enhanced Intensity → 2x brighter than Fluo-4 AM
• Better & Faster Loading → Room temperature instead of 37°C
• More Robust Results → Probe effective in a wide range of temperatures
• Variety of K_d → Many options for calcium binding dissociation constant

Fluo-8® AM Features

Fluo-8® AM is a novel fluorescent green calcium indicator that builds upon the fluorescein core while rectifying the issues that plagued earlier indicators like Fluo-3 AM and Fluo-4 AM. In particular, it has several key advantages:

• Enhanced Intensity - Tests have shown Fluo-8® AM is twice as bright as Fluo-4 AM and up to four times brighter than Fluo-3 AM
• Better Loading - Fluo-8® AM can be loaded at room temperature, unlike the 37°C incubation required by Fluo-4 AM
• Faster Loading - Room temperature loading means no need to warm up and cool down cells; finish loading in as little as 20 minutes
• Robust - Fluo-8® is less temperature dependent than other probes, leading to more consistent results
• Versatile K_d - Fluo-8® AM is available with a variety of Ca2+ binding dissociation constants, see Table 1 for details

The most brilliant achievement of Fluo-8® AM is that it improves upon the shortcomings of Fluo-3 AM and Fluo-4 AM without touching the excitation and emission. Like Fluo-3 AM and Fluo-4 AM, Fluo-8® AM will excite at ~490 nm and emit at ~520 nm. This allows for a seamless transition for those who are familiar with Fluo-3 AM and Fluo-4 AM but wish to upgrade to Fluo-8® AM. 

As with its predecessors, Fluo-8® AM is completely compatible with use in flow cytometry, fluorescence microscopy, fluorescence spectroscopy and the fluorescence microplate platform. To read more about how Fluo-8® AM has revolutionized calcium imaging, or to see additional Fluo-8® product applications, please click here. 

Table 1. Ca2+ Binding Dissociation Constants