Chromotek/F2H®-Kit Basic/代理商現(xiàn)貨

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F2H®-Kit Basic allows effortless imaging of interactions between GFP- and RFP-tagged proteins. Study your proteins of interest and domains thereof in live mammalian cells. You just need your GFP-bait and RFP-prey plasmids ready for transfection.

　　F2H® principle

　　F2H® cells (BHK origin) carry a GFP-anchoring platform in the nucleus

　　GFP- and RFP-fusion proteins (bait and prey) are transiently expressed in F2H® cells

　　GFP-bait is recruited to the platform → bright green spot

　　If RFP-prey interacts with the bait → red fluorescent spot co-localizes with the green spot

　　If RFP-prey does not interact with the bait → no red spot, disperse red fluorescence

Chromotek/F2H®-Kit Basic/代理商現(xiàn)貨

Chromotek/F2H®-Kit Basic/代理商現(xiàn)貨　　

F2H® is easy

　　You get the F2H® cell line

　　You get the Platform Reagent

　　You have your GFP-bait and RFP-prey plasmids

　　Day 1: Transfect the bait and the prey plasmids with the Platform Reagent into the F2H® cells

　　Day 2: Image and quantify your results